DGK-iota Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06558

Novus Biologicals, part of Bio-Techne
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NBP3-06558-100ul
NBP3-06558-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DGK-iota Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human DGK-iota fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DGK-iota Antibody

Immunocytochemistry/ Immunofluorescence: DGK-iota Antibody [NBP3-06558]

Immunocytochemistry/ Immunofluorescence: DGK-iota Antibody [NBP3-06558]

Immunocytochemistry/Immunofluorescence: DGK-iota Antibody [NBP3-06558] - Staining of DGKI in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human DGKI polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
Immunohistochemistry-Paraffin: DGK-iota Antibody [NBP3-06558]

Immunohistochemistry-Paraffin: DGK-iota Antibody [NBP3-06558]

Immunohistochemistry-Paraffin: DGK-iota Antibody [NBP3-06558] - Staining of human DGKI in human hepatoma with rabbit polyclonal antibody at 1:100 dilution.

Applications for DGK-iota Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DGK-iota

Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-alpha (80 kDa), -beta (90 kDa), and - gamma (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-delta (130 kDa) and DGK-

Long Name

Diacylglycerol Kinase, iota 64kDa

Alternate Names

DGKI, DGKiota

Gene Symbol

DGKI

Additional DGK-iota Products

Product Documents for DGK-iota Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DGK-iota Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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