DHPS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82649

Novus Biologicals, part of Bio-Techne
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NBP1-82649
NBP1-82649-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DHPS Antibodies »

Product Summary for DHPS Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKEINNPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYKNPGLVLDIVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHPS Antibody

Western Blot: DHPS Antibody [NBP1-82649]

Western Blot: DHPS Antibody [NBP1-82649]

Western Blot: DHPS Antibody [NBP1-82649] - Analysis in control (vector only transfected HEK293T lysate) and DHPS over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: DHPS Antibody [NBP1-82649]

Immunocytochemistry/ Immunofluorescence: DHPS Antibody [NBP1-82649]

Immunocytochemistry/Immunofluorescence: DHPS Antibody [NBP1-82649] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82649]

Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82649]

Immunohistochemistry-Paraffin: DHPS Antibody [NBP1-82649] - Staining of human prostate shows moderate to strong cytoplasmic positivity in smooth muscle cells.
View 3 more images

Applications for DHPS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHPS

The unusual amino acid hypusine is formed posttranslationally and is only found in a single cellular protein, eukaryotic translation initiation factor 5A. In the first step of hypusine biosynthesis, deoxyhypusine synthase catalyzes the NAD-dependent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the EIF5A precursor protein to form the intermediate deoxyhypusine residue. This gene consists of nine exons spanning 6.6 kb. Three transcript variants have been isolated. However, only transcript variant 1 encodes an active protein. The shorter variants may act as modulating factors of DHPS activity.

Alternate Names

deoxyhypusine synthase, DHS, DS, EC 2.5.1, EC 2.5.1.46, hypusine, MIG13, migration-inducing gene 13

Gene Symbol

DHPS

Additional DHPS Products

Product Documents for DHPS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DHPS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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