DHX37 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40580

Novus Biologicals, part of Bio-Techne
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NB110-40580
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all DHX37 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human DEAH (Asp-Glu-Ala-His) Box Protein 37 using the numbering given in entry NP_116045.2 (GeneID 57647).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX37 Antibody

Western Blot: DHX37 Antibody [NB110-40580]

Western Blot: DHX37 Antibody [NB110-40580]

Western Blot: DHX37 Antibody [NB110-40580] - Detection of human DHX37 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-DHX37 antibody NB110-40580 (lot NB110-40580-2) used for IP at 3 ug per reaction. DHX37 was also immunoprecipitated by a previous lot of this antibody (lot NB110-40580-1) and another rabbit anti-DHX37 antibody. For blotting immunoprecipitated DHX37, NB110-40580 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: DHX37 Antibody [NB110-40580]

Western Blot: DHX37 Antibody [NB110-40580]

Western Blot: DHX37 Antibody [NB110-40580] - Detection of human DHX37 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX37 antibody NB110-40580 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DHX37 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DHX37

This gene encodes a DEAD box protein. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Alternate Names

DDX37, DEAD/DEAH box helicase DDX37, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 37, DEAH (Asp-Glu-Ala-His) box polypeptide 37, DEAH box protein 37, EC 3.6.1, EC 3.6.4.13, FLJ41974, KIAA1517MGC46245, MGC2695, MGC4322, probable ATP-dependent RNA helicase DHX37

Entrez Gene IDs

57647 (Human); 208144 (Mouse)

Gene Symbol

DHX37

UniProt

NP_116045.2 (Human)

Additional DHX37 Products

Product Documents for DHX37 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DHX37 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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