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DHX58 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85348

Novus Biologicals, part of Bio-Techne
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NBP1-85348
NBP1-85348-25ul

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AINHVLQLCANLDTWCIMSPQNCCPQLQEHSQQPCKQYNLCHRRSQDPFGDLLKKLMDQIHDHLEMPELSRKFGTQMYEQQVVKLS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DHX58 Antibody

Western Blot: DHX58 Antibody [NBP1-85348]

Western Blot: DHX58 Antibody [NBP1-85348]

Western Blot: DHX58 Antibody [NBP1-85348] - (A) HeLa and (B) C2C12 cells were treated with the indicated siRNAs for 2 days and cellular lysates were subjected to Western blot analysis using anti-rapsyn antibody. GAPDH was used as loading control. Rapsyn protein levels are reduced in NUP88-depleted cells. Quantification of the respective expression levels of rapsyn after transfection of HeLa and C2C12 cells with the indicated siRNAs and shRNA-mediated depletion of NUP88 in C2C12 cells. Blots from three independent experiments for each condition were analyzed. Data present mean +/- SEM. P-values: ***<0.001; **<0.01, *<0.05; t-test, one-tailed. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1007845) licensed under a CC-BY license.
Immunohistochemistry: DHX58 Antibody [NBP1-85348]

Immunohistochemistry: DHX58 Antibody [NBP1-85348]

Immunohistochemistry: DHX58 Antibody [NBP1-85348] - Bright-field images of histological muscle sections from individual B.II.2 and a control fetus stained with anti-rapsyn antibodies (brown). Nuclei were visualized by hematoxylin. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1007845) licensed under a CC-BY license.
Western Blot: DHX58 Antibody [NBP1-85348]

Western Blot: DHX58 Antibody [NBP1-85348]

Western Blot: DHX58 Antibody [NBP1-85348] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-163

Applications for DHX58 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DHX58

Negative regulator of host innate immune defense against viruses. The repressor domain of DHX58 interactswith DDX58 and negatively regulates DDX58-mediated signaling. Binds dsRNA produced during viral replication, inparticuliar HCV RNA. Binding occurs at blunt dsRNA termini

Alternate Names

D11lgp2e, D11LGP2probable ATP-dependent RNA helicase DHX58, DEXH (Asp-Glu-X-His) box polypeptide 58, EC 3.6.4.13, LGP2ortholog of mouse D11lgp2, Probable ATP-dependent helicase LGP2, Protein D11Lgp2 homolog, RIG-I-like receptor LGP2, RLR, RNA helicase LGP2

Gene Symbol

DHX58

Additional DHX58 Products

Product Documents for DHX58 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DHX58 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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