Key Product Details

Species Reactivity

Mouse, Rat

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # V78 P3C10-D3

Concentration

1 mg/ml

View all DIAPH-2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide of Human DIAPH2

Localization

Endosome|Earlyendosome|Cytosol

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for DIAPH-2 Antibody (V78 P3C10-D3)

Western Blot: DIAPH-2 Antibody (V78 P3C10-D3) [NBP3-18260]

Western Blot: DIAPH-2 Antibody (V78 P3C10-D3) [NBP3-18260]

Western Blot: DIAPH-2 Antibody (V78 P3C10-D3) [NBP3-18260] - Western Blot analysis of Mouse liver showing detection of 125 kda DIAPH-2 protein using Mouse Anti-DIAPH-2 Monoclonal Antibody, Clone V78 P3C10-D3 (NBP3-18260). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver (10ug). Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-DIAPH-2 Monoclonal Antibody (NBP3-18260) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 125 kda.

Applications for DIAPH-2 Antibody (V78 P3C10-D3)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Optimal dilutions for assays should be determined by the user.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS pH 7.4, 50% glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DIAPH-2

DIAPH2 may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described.

Long Name

Diaphanous Homolog 2

Alternate Names

DIA2, DIAPH2, DRF2, POF2

Gene Symbol

DIAPH2

Additional DIAPH-2 Products

Product Documents for DIAPH-2 Antibody (V78 P3C10-D3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DIAPH-2 Antibody (V78 P3C10-D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.