Dicer Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-71691

Novus Biologicals, part of Bio-Techne
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NBP1-71691-0.1mg
NBP1-71691SS
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Dicer Antibodies »

Product Specifications

Immunogen

A recombinant protein made to an internal region of the human Dicer protein (within residues 1100-1300). [Swiss-Prot# Q9UPY3]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%) and Chinese Hamster (82%).

Localization

Cytoplasm.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dicer Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Dicer Antibody - BSA Free [NBP1-71691]

Immunocytochemistry/ Immunofluorescence: Dicer Antibody - BSA Free [NBP1-71691]

Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Dicer at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: Dicer Antibody - BSA Free [NBP1-71691]

Immunohistochemistry-Paraffin: Dicer Antibody - BSA Free [NBP1-71691]

Immunohistochemistry-Paraffin: Dicer Antibody [NBP1-71691] - Analysis of Dicer in paraffin embedded human colorectal cancer tissue using anti-Dicer antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Dicer Antibody - BSA Free [NBP1-71691]

Immunocytochemistry/ Immunofluorescence: Dicer Antibody - BSA Free [NBP1-71691]

Immunocytochemistry/Immunofluorescence: Dicer Antibody [NBP1-71691] - Dicer antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
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Applications for Dicer Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-5 ug/ml

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

2.0 ug/ml
Application Notes
This Dicer antibody is useful for for Immunocytochemistry/Immunofluorescence and Immunohistochemistry-paraffin embedded sections. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed in the cytoplasm of mouse prostate. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP1-71691 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dicer

Dicer (DICER1 or double-strand-specific ribonuclease mDCR-1) belongs to RNase III family and is essential for RNA induced silencing complex (RISC) formation and it also forms a component of RISC loading complex (RLC) also known as the miRNA loading complex (miRLC) that contains - DICER1, EIF2C2/AGO2 and TARBP2. Within RLC/miRLC complex, DICER1 and TARBP2 are required to process precursor miRNAs to mature miRNAs and then load them onto EIF2C2/AGO2. Dicer also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which targets selective destruction of complementary RNAs. siRNAs play a key roles in mitosis and DICER1 mutations have been shown to be associated with malignancies including blastoma, breast cancer, adenocarcinoma, and pancreatitis. It is also involved in myelination and other nervous system protections as well as pathologies including miRNA induced malformations of critical sensory cells in vision/hearing, cardiovascular diseases etc.

Alternate Names

DCR1, Dicer, dicer 1, double-stranded RNA-specific endoribonuclease, dicer 1, ribonuclease type III, Dicer1, Dcr-1 homolog (Drosophila), EC 3.1.26, EC 3.1.26.-, EC 3.1.26.3, Helicase MOI, Helicase with RNase motif, helicase-moi, HERNADicer1, Dcr-1 homolog, K12H4.8-LIKE, KIAA0928endoribonuclease Dicer

Entrez Gene IDs

23405 (Human); 192119 (Mouse)

Gene Symbol

DICER1

UniProt

Q9UPY3 (Human), Q8R418 (Mouse)

Additional Dicer Products

Product Documents for Dicer Antibody - BSA Free

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Certificate of Analysis

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Product Specific Notices for Dicer Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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