Dihydrofolate Reductase/DHFR Antibody (9B2)

Western Blot: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Rat heart tissue, Mouse kidney tissue, Mouse liver tissue. All lanes: Dihydrofolate Reductase/DHFR antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 22, 16 KDa. Observed band size: 22 KDa.

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - IHC image of Dihydrofolate Reductase/DHFR antibody diluted at 1:100 and staining in paraffin-embedded human liver cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (9B2) [NBP3-15195] - IHC image of Dihydrofolate Reductase/DHFR antibody diluted at 1:100 and staining in paraffin-embedded human breast cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.