Dihydrofolate Reductase/DHFR Antibody (JE31-05)

Western Blot: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268]

Western Blot: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268] - Western blot analysis of Dihydrofolate Reductase/DHFR on different lysates with Rabbit anti-Dihydrofolate Reductase/DHFR antibody (NBP3-32268) at 1/1,000 dilution.

Lane 1: HCT116 cell lysate (15 ug/Lane)
Lane 2: HeLa cell lysate (15 ug/Lane)
Lane 3: U-2 OS cell lysate (15 ug/Lane)
Lane 4: K-562 cell lysate (15 ug/Lane)
Lane 5: MCF7 cell lysate (15 ug/Lane)
Lane 6: HEK-293 cell lysate (15 ug/Lane)
Lane 7: Jurkat cell lysate (15 ug/Lane)
Lane 8: HepG2 cell lysate (15 ug/Lane)
Lane 9: LO2 cell lysate (15 ug/Lane)
Lane 10: Mouse liver tissue lysate (30 ug/Lane)
Lane 11: Rat liver tissue lysate (30 ug/Lane)

Predicted band size: 21.5 kDa
Observed band size: 21.5 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32268) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268]

Immunohistochemistry: Dihydrofolate Reductase/DHFR Antibody (JE31-05) [NBP3-32268] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Dihydrofolate Reductase/DHFR antibody (NBP3-32268) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32268) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.