Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70574

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6D5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Dihydrolipoamide Dehydrogenase/DLD Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DLD(NP_000099) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5)Azide and BSA Free [NBP2-70574]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5)Azide and BSA Free [NBP2-70574]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Lipoamide Dehydrogenase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with ant
Immunocytochemistry/ Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574]

Immunocytochemistry/ Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574]

Immunocytochemistry/Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Lipoamide Dehydrogenase.
Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574]

Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574]

Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (OTI6D5) - Azide and BSA Free [NBP2-70574] - Staining of paraffin-embedded Human lymph node tissue using anti-Lipoamide Dehydrogenase mouse monoclonal antibody.
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Applications

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dihydrolipoamide Dehydrogenase/DLD

Lipoamide Dehydrogenase encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Alternate Names

Diaphorase, DLD, DLDD, DLDH, GCSL, LAD, Lipoamide Dehydrogenase, PHE3

Gene Symbol

DLD

Additional Dihydrolipoamide Dehydrogenase/DLD Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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