Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83)

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539]

Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Western blot analysis of Dihydrolipoamide Dehydrogenase/DLD on different lysates with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/1,000 dilution.

Lane 1: Jurkat cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: 293T cell lysate (20 ug/Lane)
Lane 4: MCF7 cell lysate (20 ug/Lane)
Lane 5: K-562 cell lysate (20 ug/Lane)
Lane 6: SK-Br-3 cell lysate (20 ug/Lane)
Lane 7: U-937 cell lysate (20 ug/Lane)
Lane 8: HepG2 cell lysate (20 ug/Lane)
Lane 9: NIH/3T3 cell lysate (20 ug/Lane)
Lane 10: Mouse liver tissue lysate (40 ug/Lane)

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32539) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539]

Immunohistochemistry: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32539) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539]

Immunocytochemistry/ Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody (PSH0-83) [NBP3-32539] - Immunocytochemistry analysis of HeLa cells labeling Dihydrolipoamide Dehydrogenase/DLD with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/200 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Dihydrolipoamide Dehydrogenase/DLD antibody (NBP3-32539) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.