Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-916

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence TCCSVLINKKVDS corresponding to C-Terminus according to NP_036269.1, NP_001127917.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Staining of Human Cerebellum (A), Kidney (B) and Testes (C) lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Immunocytochemistry/ Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Immunocytochemistry/Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic, nuclear and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Flow Cytometry: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916]

Flow Cytometry: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NB300-916] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Applications for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:1000

Western Blot

0.1 - 0.3 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dimethylarginine Dimethylaminohydrolase 1/DDAH1

This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Alternate Names

DDAHI, Dimethylargininase-1

Entrez Gene IDs

23576 (Human)

Gene Symbol

DDAH1

UniProt

NP_036269.1 (Human)

Additional Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Products

Product Documents for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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