Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85273

Novus Biologicals, part of Bio-Techne
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NBP1-85273
NBP1-85273-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SKRTNQRGAEILADTFKDYAVSTVPVADGLHLKSFCSMAGPNLIAIGSSESAQKALKIMQQMSDHRYDKLTVPDDIAANCIYLNIPNKGHVLLHRTPEEYPESAKVYEKLKDHMLIPVSMSELEKVDGLLT

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 22440215).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273]

Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273]

Immunohistochemistry-Paraffin: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining in human kidney and skin tissues using anti-DDAH1 antibody. Corresponding DDAH1 RNA-seq data are presented for the same tissues.
Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273]

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273]

Western Blot: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273]

Immunocytochemistry/ Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273]

Immunocytochemistry/Immunofluorescence: Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody [NBP1-85273] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.
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Applications for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 22440215). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dimethylarginine Dimethylaminohydrolase 1/DDAH1

DDAH1 belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

Alternate Names

DDAHI, Dimethylargininase-1

Gene Symbol

DDAH1

Additional Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Products

Product Documents for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dimethylarginine Dimethylaminohydrolase 1/DDAH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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