DIO3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-05767

Novus Biologicals, part of Bio-Techne
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NBP1-05767
NBP1-05767SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster, Rabbit

Cited:

Human, Mouse, Rat, Hamster

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all DIO3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of rat DIO3 (within residues 250-300). [Swiss-Prot# P49897]

Reactivity Notes

Rabbit reactivity via customer review.

Localization

Cell membrane; Single-pass type II membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DIO3 Antibody - BSA Free

Western Blot: DIO3 AntibodyBSA Free [NBP1-05767]

Western Blot: DIO3 AntibodyBSA Free [NBP1-05767]

Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rat placenta.
Flow Cytometry: DIO3 Antibody - BSA Free [NBP1-05767]

Flow Cytometry: DIO3 Antibody - BSA Free [NBP1-05767]

Flow Cytometry: DIO3 Antibody [NBP1-05767] - Analysis using the Biotin conjugate of NBP1-05767. Dio3 staining of mouse hepatic stellate cell cultured for 7 days. Image from verified customer review.
Western Blot: DIO3 AntibodyBSA Free [NBP1-05767]

Western Blot: DIO3 AntibodyBSA Free [NBP1-05767]

Western Blot: DIO3 Antibody [NBP1-05767] - Detection of Dio3 in rabbit brain whole cell homogenate. Photo courtesy of product review by verified customer.

Applications for DIO3 Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported by customer review using biotinylated antibody

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 24885572)

Immunohistochemistry

reported in scientific literature (PMID 24174657)

Immunohistochemistry-Frozen

reported in scientific literature (PMID 24885572)

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 25004090)

Western Blot

0.5 ug/ml
Application Notes
In Western blot, a band is seen ~31 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 3.5 using NBP1-05767 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DIO3

DIO3 (type III iodothyronine deiodinase), an iodothyronine deiodinase family member, is expressed in placenta and several fetal tissues in the plasma/endosomal membranes as single-pass type II membrane protein, and it catalyzes the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine), both RT3 and T2 being inactive metabolites thus preventing premature exposure of developing fetus to adult levels of thyroid hormones. DIO3 controls circulating thyroid hormone levels throughout gestation by controlling thyroid hormone inactivation during embryological development. DIO3 is homodimeric and may undergo minor heretodimerization with DIO1 or DIO2, homo- or heterodimer state being critical for its catalytic activity. Its activity has been identified in only a limited number of postnatal tissues, i.e. brain, skin, and pregnant uterus, whereas it is abundantly present in several fetal tissues. Furthermore, DIO3 re-expression has been shown in certain pathologies that include cancer, cardiac hypertrophy, myocardial infarction, chronic inflammation, and critical illness.

Alternate Names

5DIII, D3, deiodinase, iodothyronine, type III, DIOIII, EC 1.97.1, EC 1.97.1.11, ITDI3, placental type, thyroxine deiodinase type III (selenoprotein), Type 3 DI, type 3 iodothyronine selenodeiodinase, type III iodothyronine deiodinase, type-III 5' deiodinase, Type-III 5'-deiodinase

Gene Symbol

DIO3

Additional DIO3 Products

Product Documents for DIO3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DIO3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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