DIS3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85209

Novus Biologicals, part of Bio-Techne
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NBP1-85209
NBP1-85209-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DIS3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSN

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24150935), Rat (87%), Mouse (86%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DIS3 Antibody

Western Blot: DIS3 Antibody [NBP1-85209]

Western Blot: DIS3 Antibody [NBP1-85209]

Western Blot: DIS3 Antibody [NBP1-85209] - Analysis in U-87MG ATCC cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: DIS3 Antibody [NBP1-85209]

Western Blot: DIS3 Antibody [NBP1-85209]

Western Blot: DIS3 Antibody [NBP1-85209] - Analysis in human cell line U-87 MG.
Immunocytochemistry/ Immunofluorescence: DIS3 Antibody [NBP1-85209]

Immunocytochemistry/ Immunofluorescence: DIS3 Antibody [NBP1-85209]

Immunocytochemistry/Immunofluorescence: DIS3 Antibody [NBP1-85209] - Staining of human cell line U-2 OS shows localization to nucleus & cytosol. Antibody staining is shown in green
View 4 more images

Applications for DIS3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DIS3

DIS3 is a component of the exosome 3'->5' exoribonuclease complex. Required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. Also associated with the GTPase Ran. Has a 3'-5' exonuclease activity

Alternate Names

2810028N01Rik, DIS3 mitotic control homolog (S. cerevisiae), dis3p, DKFZp667L1817, EC 3.1.13, EC 3.1.13.-, EC 3.1.26.-, EXOSC11, exosome complex exonuclease RRP44, exosome component 11, FLJ10484, KIAA1008RP11-342J4.3, MGC33035, mitotic control protein dis3 homolog, Protein DIS3 homolog, Ribosomal RNA-processing protein 44, RRP44bA555G22.1

Gene Symbol

DIS3

Additional DIS3 Products

Product Documents for DIS3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DIS3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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