Dishevelled-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32388

Novus Biologicals, part of Bio-Techne
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NBP1-32388
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Human

Predicted:

Bovine (99%), Rat (99%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dishevelled-2 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Dishevelled-2. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (80%), Xenopus laevis (85%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dishevelled-2 Antibody

Western Blot: Dishevelled-2 Antibody [NBP1-32388]

Western Blot: Dishevelled-2 Antibody [NBP1-32388]

Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Intracellular distribution of Wwox (dilution 1:1000, AF6399), Dvl-1 (dilution 1:700, NBP1-58318), Dvl-2 (Dishevelled-2, dilution 1:1000,) and Dvl-3 (dilution 1:200, NBP2-24674) proteins in HET-1A and SCC-15 cell lines. Image collected and cropped by CiteAb from the following publication (www.jcancer.org/v11p4030.htm) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Dishevelled-2 Antibody [NBP1-32388]

Immunohistochemistry-Paraffin: Dishevelled-2 Antibody [NBP1-32388]

Immunohistochemistry-Paraffin: Dishevelled-2 Antibody [NBP1-32388] - Paraffin-embedded lung SCC, using antibody at 1:100 dilution.
Western Blot: Dishevelled-2 Antibody [NBP1-32388]

Western Blot: Dishevelled-2 Antibody [NBP1-32388]

Western Blot: Dishevelled-2 Antibody [NBP1-32388] - Sample(30 ug whole cell lysate)A: MOLT4 )B: Raji 7. 5% SDS PAGE, antibody diluted at 1:500.
View 2 more images

Applications for Dishevelled-2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dishevelled-2

This gene encodes a member of the dishevelled (dsh) protein family. The vertebrate dsh proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. This gene encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein, which may play a role in the signal transduction pathway mediated by multiple Wnt proteins. The mechanisms of dishevelled function in Wnt signaling are likely to be conserved among metazoans. [provided by RefSeq]

Alternate Names

Dishevelled2, DVL2

Gene Symbol

DVL2

Additional Dishevelled-2 Products

Product Documents for Dishevelled-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dishevelled-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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