Dishevelled-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16168
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (92%), Monkey (96%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Dishevelled-2 Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Dishevelled-2. The exact sequence is proprietary.
Reactivity Notes
Rhesus Monkey (96%).
Localization
Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
79 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Dishevelled-2 Antibody
Western Blot: Dishevelled-2 Antibody [NBP2-16168]
Western Blot: Dishevelled-2 Antibody [NBP2-16168] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dishevelled-2 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Dishevelled-2 Antibody [NBP2-16168]
Immunocytochemistry/Immunofluorescence: Dishevelled-2 Antibody [NBP2-16168] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dvl-2 protein stained by Dvl-2 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.Western Blot: Dishevelled-2 Antibody [NBP2-16168]
Western Blot: Dishevelled-2 Antibody [NBP2-16168] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dishevelled 2 antibody [N1N3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibodyApplications for Dishevelled-2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Western Blot
1:1000-1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Dishevelled-2
Additional Dishevelled-2 Products
Product Documents for Dishevelled-2 Antibody
Product Specific Notices for Dishevelled-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.