DKC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-59738

Novus Biologicals, part of Bio-Techne
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NB100-59738
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all DKC1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KRKRESESESDETPP corresponding to internal region according to NP_001354.1.

Epitope

KRKRESESESDETPP

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DKC1 Antibody

Immunohistochemistry-Paraffin: DKC1 Antibody [NB100-59738]

Immunohistochemistry-Paraffin: DKC1 Antibody [NB100-59738]

Immunohistochemistry-Paraffin: DKC1 Antibody [NB100-59738] - IHC analysis of DKC1 in paraffin embedded Human Spleen using this antibody at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for DKC1 Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

5 - 10 ug/ml

Peptide ELISA

Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 70-75 kDa band in human bone marrow, duodenum and skin lysates after 0.01 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.7 kDa band according to NP_001354.1. The 70-75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human spleen shows strong nuclear staining of select splenocytes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DKC1

DKC1 (Dyskeratosis congenita 1) is a nucleolar protein, altered in dyskeratosis congenita, which carries out two separate functions, both fundamental for proliferating cells. One function is the pseudouridylation of ribosomal RNA (rRNA) molecules, necessary for their processing, and the other is the stabilization of the telomerase RNA component, necessary for telomerase activity. It is thought to be the catalytic subunit of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. DKC1 is also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. Both 18S rRNA production and rRNA pseudouridylation are impaired if DKC1 is depleted.

Alternate Names

CBF5, CBF5 homolog, cbf5p homolog, DKC, dyskeratosis congenita 1, dyskerin, Dyskerin, EC 5.4.99, EC 5.4.99.-, FLJ97620, H/ACA ribonucleoprotein complex subunit 4, NAP57, NOLA4dyskerin, Nopp140-associated protein of 57 kDa, Nucleolar protein family A member 4, Nucleolar protein NAP57, snoRNP protein DKC1, XAP101

Entrez Gene IDs

1736 (Human); 245474 (Mouse)

Gene Symbol

DKC1

UniProt

NP_001354.1 (Human)

Additional DKC1 Products

Product Documents for DKC1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DKC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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