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Dkk-1 Antibody (LIV-1205) - Humanized

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-28068

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # LIV-1205

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

DLK1

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

< 0.001EU/ug,determined by LAL method.

Description

Expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Upon receipt, store immediately at -20C or lower for 24 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Dkk-1 Antibody (LIV-1205) - Humanized

Dkk-1 Antibody (LIV-1205) - Humanized

ELISA: Dkk-1 Antibody (LIV-1205) - Humanized[NBP3-28068] -

Immobilized human DLK1.1 His at 2 ug/mL can bind Dkk-1 Antibody (LIV-1205) - Humanized, EC50=0.01281 ug/mL.
Dkk-1 Antibody (LIV-1205) - Humanized

Flow Cytometry: Dkk-1 Antibody (LIV-1205) - Humanized[NBP3-28068] -

Human DLK1 HEK293 cells were stained with Dkk-1 Antibody (LIV-1205) - Humanized and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50=0.1334 ug/mL.

Applications for Dkk-1 Antibody (LIV-1205) - Humanized

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Formulation

Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Dkk-1

DKK1 is a secreted factor that has been linked to the function of osteoblasts. Elevated DKK1 levels in bone marrow plasma and peripheral blood from patients with multiple myeloma correlated with the gene-expression patterns of DKK1 and were associated with the presence of focal bone lesions. The production of DKK1, an inhibitor of osteoblast differentiation, by myeloma cells is associated with the presence of lytic bone lesions in patients with multiple myeloma.

Long Name

Dickkopf-1

Alternate Names

Dkk1

Gene Symbol

DKK1

UniProt

Additional Dkk-1 Products

Product Documents for Dkk-1 Antibody (LIV-1205) - Humanized

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dkk-1 Antibody (LIV-1205) - Humanized

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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