DLG7/HURP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-40573

Novus Biologicals, part of Bio-Techne
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NB110-40573
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all DLG7/HURP Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 200 and 250 of Human Hepatoma Upregulated Protein/Discs, Large 7 using the numbering given in entry NP_055565.2 (GeneID 9787).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DLG7/HURP Antibody

Western Blot: DLG7/HURP Antibody [NB110-40573]

Western Blot: DLG7/HURP Antibody [NB110-40573]

Western Blot: DLG7/HURP Antibody [NB110-40573] - Detection of Human HURP/DLG7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HURP/DLG7 antibody NB110-40573 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NB110-40573]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NB110-40573]

Immunohistochemistry-Paraffin: DLG7/HURP Antibody [NB110-40573] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- HURP/DLG7 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: DLG7/HURP Antibody [NB110-40573]

Western Blot: DLG7/HURP Antibody [NB110-40573]

Western Blot: DLG7/HURP Antibody [NB110-40573] - Detection of Human HURP/DLG7 on HeLa whole cell lysate using NB110-40573. HURP/DLG7 was also immunoprecipitated by rabbit anti-HURP/DLG7 antibodies NB110-40572 and NB110-40574.

Applications for DLG7/HURP Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DLG7/HURP

HURP is a novel component of the Ran-importin spindle assembly pathway. HURP binds microtubules and affects their organization both in vitro and in vivo. In egg extract, anti-HURP antibodies disrupt the formation of both Ran-dependent and chromatin and centrosome-induced spindles. HURP is also required for the proper formation and function of mitotic spindles in HeLa cells. HURP is also over-expressed in some tumor types

Alternate Names

DAP-5, Discs large homolog 7, discs, large (Drosophila) homolog-associated protein 5, disks large-associated protein 5, DLG7, Hepatoma up-regulated protein, HURPDLG1, KIAA0008discs, large homolog 7 (Drosophila), large homolog 7

Entrez Gene IDs

9787 (Human)

Gene Symbol

DLGAP5

UniProt

NP_055565.2 (Human)

Additional DLG7/HURP Products

Product Documents for DLG7/HURP Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DLG7/HURP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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