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DLX5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28911

Novus Biologicals, part of Bio-Techne
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NBP1-28911

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-AYNRVPSATNQPEK corresponding to internal region according to NP_005212.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for DLX5 Antibody

Western Blot: DLX5 Antibody [NBP1-28911]

Western Blot: DLX5 Antibody [NBP1-28911]

Western Blot: Dlx5 Antibody [NBP1-28911] - (1ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-28911]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-28911]

Immunohistochemistry-Paraffin: DLX5 Antibody [NBP1-28911] - (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for DLX5 Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
Western blot: Approx 30kDa band observed in Human Bone Marrow lysates (calculated MW of 31.5kDa according to NP_005212.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, J Biol Chem. 2003 Sep 5;278(36):34387-94; PMID: 12815054).Primary incubation was 1 hour.
IHC: Paraffin embedded Human Placenta.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DLX5

This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein may play a role in bone development and fracture healing. Mutation in this gene, which is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 7, may be associated with split-hand/split-foot malformation. [provided by RefSeq]

Long Name

Distal-less Homeobox 5

Alternate Names

distal-less homeo box 5, distal-less homeobox 5, homeobox protein DLX-5

Entrez Gene IDs

1749 (Human); 13395 (Mouse); 25431 (Rat)

Gene Symbol

DLX5

Additional DLX5 Products

Product Documents for DLX5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DLX5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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