Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

1.0 mg/ml

View all DMAP1 Antibodies »

Product Summary for DMAP1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human DNA Methyltransferase 1 Associated Protein 1 using the numbering given in entry NP_061973.1 (GeneID 55929).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DMAP1 Antibody

Western Blot: DMAP1 Antibody [NB100-256]

Western Blot: DMAP1 Antibody [NB100-256]

Western Blot: DMAP1 Antibody [NB100-256] - Detection of Human DMAP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DMAP1 antibody NB100-256 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: DMAP1 Antibody [NB100-256]

Western Blot: DMAP1 Antibody [NB100-256]

Western Blot: DMAP1 Antibody [NB100-256] - Detection of Human DMAP1 by Western Blot. Sample: Whole cell extract (50 mcg/lane) from HeLa cells. Antibody: Affinity purified goat anti-DMAP1 (Cat. No. NB100-256) used at the concentrations that are indicated. Detection: Chemiluminescence with 2 minute exposure.

Applications for DMAP1 Antibody

Application
Recommended Usage

Western Blot

1:1000-1:5000
Application Notes
By Western blot, this antibody recognizes bands around 60 kDa representing DMAP1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DMAP1

DMAP1 (DNA methyltransferase 1 associated protein 1) has been identified as a subunit of several distinct protein complexes involved in the repression and activation of transcription. It has been observed to interact with histone deacetylase 2 (HDAC2) as well as histone acetyltransferase (HAT) indicating a role in nucleosome modification to regulate transcription. DMAP1 can act independently to repress transcription in association with DNA methyltransferase 1. Alternate names for DMAP1 include DNMT1-associated protein 1, DNMAP1, DMAP1, KIAA1425, EAF2, SWC4, DNMAP1, DNMTAP1, FLJ11543, and DKFZp686L09142.

Alternate Names

DNA methyltransferase 1 associated protein 1, DNA methyltransferase 1-associated protein 1, DNMAP1DNMT1-associated protein 1, DNMT1 associated protein 1, DNMTAP1, EAF2, FLJ11543, KIAA1425DKFZp686L09142, MEAF2, SWC4

Entrez Gene IDs

55929 (Human)

Gene Symbol

DMAP1

UniProt

Q9NPF5 (Human)

Additional DMAP1 Products

Product Documents for DMAP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DMAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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