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Key Product Details

Species Reactivity

Human, Mouse, Bovine

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 2H12/4

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Human DMC1 protein (expressed as 6xHis fusion in E. coli and band cut out of gel of insoluble pelet)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

Specific for DMC1 - does not cross-react with the related protein Rad51.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for DMC1 Antibody (2H12/4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Predicted molecular weight: 41 kDa. Optimal dilutions/concentrations should be determined by the end user. Unstable after acidic elution.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DMC1

The DMC1 protein is essential for meiotic homologous recombination. Genetic recombination in meiosis plays an important role in generating diversity of genetic information. The product of this gene is structurally and evolutionary related to the products of the yeast RAD51 and E. coli RecA genes.

Alternate Names

disrupted meiotic cDNA1, yeast, homolog of, DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologousrecombination (yeast), DMC1 homologue, HsLim15, LIM15yeast homolog) meiosis-specific homologousrecombination, meiotic recombination protein DMC1/LIM15 homolog, MGC150472, MGC150473

Entrez Gene IDs

11144 (Human); 13404 (Mouse); 362960 (Rat)

Gene Symbol

DMC1

Additional DMC1 Products

Product Documents for DMC1 Antibody (2H12/4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DMC1 Antibody (2H12/4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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