DNA Ligase I Antibody (10H5)

Name: DNA Ligase I Antibody (10H5)
Brand: Novus Biologicals
SKU: NB100-119

Novus Biologicals, part of Bio-Techne | Catalog # NB100-119

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Citations (19)

Product Datasheet / COA / SDS

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Avian - Chicken, Bacteria, Fish, Hamster, Rabbit

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Radioimmunoassay, Western Blot

Cited:

Bacteria, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Mass Spectrometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 10H5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

Full-length recombinant human DNA Ligase I protein

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.. Rabbit reactivity reported in scientific literature (PMID: 12668657). Hamster reactivity reported in scientific literature (PMID: 11912211). Fish, and chicken reactivity reported in scientific literature (PMID: 12928478). Bacteria reactivity reported in scientific literature (PMID: 9603940).

Localization

Nuclear

Specificity

This is specific for DNA ligase 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DNA Ligase I Antibody (10H5)

Western Blot: DNA Ligase I Antibody (10H5) [NB100-119]

Western Blot: DNA Ligase I Antibody (10H5) [NB100-119] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: DNA Ligase I Antibody (10H5) [NB100-119]

Immunocytochemistry/Immunofluorescence: DNA Ligase I Antibody (10H5) [NB100-119] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.

Immunohistochemistry-Paraffin: DNA Ligase I Antibody (10H5) [NB100-119]

Immunohistochemistry-Paraffin: DNA Ligase I Antibody (10H5) [NB100-119] - Human breast carcinoma. DNA ligase I stained by DNA ligase I antibody [10H5] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.