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DNA Ligase I Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55992

Novus Biologicals, part of Bio-Techne
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NBP2-55992
NBP2-55992-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: RNQEDNTGKYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVREPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA Ligase I Antibody

Immunocytochemistry/ Immunofluorescence: DNA Ligase I Antibody [NBP2-55992]

Immunocytochemistry/ Immunofluorescence: DNA Ligase I Antibody [NBP2-55992]

Immunocytochemistry/Immunofluorescence: DNA Ligase I Antibody [NBP2-55992] - Staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.

Applications for DNA Ligase I Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA Ligase I

DNA ligase 1 catalyzes the joining of double-stranded DNA ends during replication, recombination, and repair. Structurally, DNA ligase 1 is toroidal and encircles nicked DNA substrates. The 9-1-1 complex, a DNA damage checkpoint complex, has been reported to associate with DNA ligase 1 and stimulate ligase activity. Mutations in DNA ligase 1 result in immunodeficiency and increased sensitivity to DNA-damaging agents.

Long Name

DNA ligase 1

Alternate Names

EC 6.5.1.1

Gene Symbol

LIG1

Additional DNA Ligase I Products

Product Documents for DNA Ligase I Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNA Ligase I Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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