DNA Polymerase beta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05939

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DNA Polymerase beta Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human DNA Polymerase beta fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DNA Polymerase beta Antibody

Western Blot: DNA Polymerase beta Antibody [NBP3-05939]

Western Blot: DNA Polymerase beta Antibody [NBP3-05939]

Western Blot: DNA Polymerase beta Antibody [NBP3-05939] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:37 kDaObserved band size:40 kDa
Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-05939]

Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-05939]

Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-05939] - Staining of human POLB in human testis with rabbit polyclonal antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-05939]

Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-05939]

Immunohistochemistry-Paraffin: DNA Polymerase beta Antibody [NBP3-05939] - Staining of human POLB in human gallbladder with rabbit polyclonal antibody at 1:500 dilution.
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Applications for DNA Polymerase beta Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNA Polymerase beta

DNA polymerase beta comprises an amino terminal 8kDa domain and a carboxy terminal 31kDa domain. DNA Polymerase beta, a DNA repair polymerase is constitutively expressed in cultured cells. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.

Alternate Names

POLB

Gene Symbol

POLB

Additional DNA Polymerase beta Products

Product Documents for DNA Polymerase beta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNA Polymerase beta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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