DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25609
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2150
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fragment of human DNA Polymerase epsilon catalytic subunit A.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images
Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free [NBP3-25609] -
Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free [NBP3-25609] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody [HL2150] (NBP3-25609) diluted at 1:900. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free [NBP3-25609] -
Immunohistochemistry-Paraffin: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free [NBP3-25609] - POLE antibody [HL2150] detects POLE protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. POLE stained by POLE antibody [HL2150] (NBP3-25609) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free [NBP3-25609] -
Western Blot: DNA Polymerase epsilon catalytic subunit A Antibody (HL2150) - Azide and BSA Free [NBP3-25609] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with POLE antibody [HL2150] (NBP3-25609) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.Applications
Application
Recommended Usage
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DNA Polymerase epsilon catalytic subunit A
Alternate Names
DKFZp434F222, DNA polymerase epsilon catalytic subunit A, DNA polymerase II subunit A, EC 2.7.7, EC 2.7.7.7, POLE1FLJ21434, polymerase (DNA directed), epsilon
Gene Symbol
POLE
Additional DNA Polymerase epsilon catalytic subunit A Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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