DNA Polymerase lambda Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81665

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all DNA Polymerase lambda Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 525 and 575 of human polymerase (DNA directed), lambda using the numbering given in entry NP_037406.1 (GeneID 27343).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNA Polymerase lambda Antibody - BSA Free

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665]

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665]

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665] - Detection of human Pol Lambda by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Pol Lambda (NB100-81665). Detection: DAB
Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665]

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665]

Immunohistochemistry-Paraffin: DNA Polymerase lambda Antibody [NB100-81665] - Detection of mouse Pol Lambda by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti- Pol Lambda (NB100-81665). Detection: DAB
Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81665]

Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81665]

Immunoprecipitation: DNA Polymerase lambda Antibody [NB100-81665] - Detection of human Pol Lambda by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Pol Lambda antibody NB100-81665 used for IP at 6 ug per reaction. Pol Lambda was also immunoprecipitated by rabbit anti-Pol Lambda antibody NB100-81664. For blotting immunoprecipitated Pol Lambda, NB100-81665 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for DNA Polymerase lambda Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Western Blot Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DNA Polymerase lambda

DNA polymerase lambda (pol lambda) is a DNA polymerase involved in base excision repair (BER). Pol lambda possesses terminal transferase and 5'-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A. Alternate names for pol lambda include DNA polymerase kappa, DNA polymerase beta-2, pol beta-2, POLL, POLL-KAPPA, and BETA-N.

Alternate Names

BETAN, DNA polymerase beta-2, DNA polymerase beta-N, DNA polymerase kappa, DNA polymerase lambda, EC 2.7.7.7, EC 4.2.99.-, FLJ46002, Pol beta2, Pol Lambda, POLKAPPA, polymerase (DNA directed), lambda

Entrez Gene IDs

27343 (Human)

Gene Symbol

POLL

UniProt

NP_037406.1 (Human)

Additional DNA Polymerase lambda Products

Product Documents for DNA Polymerase lambda Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNA Polymerase lambda Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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