DNAJC6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81507

Novus Biologicals, part of Bio-Techne
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NBP1-81507
NBP1-81507-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DNAJC6 Antibodies »

Product Summary for DNAJC6 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NGDKPHGVKKPSKKQQEPAAPPPPEDVDLLGLEGSAMSNSFSPPAAPPTNSELLSDLFGGGGAAGPTQAGQSGVEDVFHPSGPASTQSTP

Reactivity Notes

Mouse reactivity reported in scientific literature (Roosen et al). Rat (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNAJC6 Antibody

Immunohistochemistry-Paraffin: DNAJC6 Antibody [NBP1-81507]

Immunohistochemistry-Paraffin: DNAJC6 Antibody [NBP1-81507]

Immunohistochemistry-Paraffin: DNAJC6 Antibody [NBP1-81507] - Analysis in human cerebral cortex and pancreas tissues using Anti-DNAJC6 antibody. Corresponding DNAJC6 RNA-seq data are presented for the same tissues.
Western Blot: DNAJC6 Antibody [NBP1-81507]

Western Blot: DNAJC6 Antibody [NBP1-81507]

Western Blot: DNAJC6 Antibody [NBP1-81507] - Analysis in human cell line HEL.
Immunocytochemistry/ Immunofluorescence: DNAJC6 Antibody [NBP1-81507]

Immunocytochemistry/ Immunofluorescence: DNAJC6 Antibody [NBP1-81507]

Immunocytochemistry/Immunofluorescence: DNAJC6 Antibody [NBP1-81507] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
View 2 more images

Applications for DNAJC6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DNAJC6

DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]

Alternate Names

DJC6, DnaJ (Hsp40) homolog, subfamily B, member 6, DnaJ (Hsp40) homolog, subfamily C, member 6, DnaJ homolog subfamily C member 6, EC 3.1.3.48, KIAA0473auxilin, MGC129914, MGC129915, MGC48436, putative tyrosine-protein phosphatase auxilin

Gene Symbol

DNAJC6

Additional DNAJC6 Products

Product Documents for DNAJC6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DNAJC6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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