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DNMT1 Antibody (1A9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26614

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26614-100ul
NBP3-26614-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1A9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human DNMT1 [UniProt P26358]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DNMT1 Antibody (1A9)

DNMT1 Antibody (1A9)

Immunohistochemistry: DNMT1 Antibody (1A9) [NBP3-26614] -

Immunohistochemistry: DNMT1 Antibody (1A9) [NBP3-26614] - Image of DNMT1 Antibody (1A9) diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
DNMT1 Antibody (1A9)

Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (1A9) [NBP3-26614] -

Immunocytochemistry/Immunofluorescence: DNMT1 Antibody (1A9) [NBP3-26614] - Staining of HepG2 Cells with DNMT1 Antibody (1A9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for DNMT1 Antibody (1A9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: DNMT1

DNA (cytosine-5)-methyltransferase 1 (DNMT1) plays a role in the regulation of tissue-specific cytosine residue methylation patterns that are established during development. Spec ifically, DNMT-1 interacts with Histone deacetylases and other methyltransferases to preferentially methylate hemimethylated DNA in coordination with the methylation of histones.

Improper methylation patterns have been linked to certain human tumors and developmental abnormalities. DNMT1 antibodies are useful tools for DNA methlyation research and studies on certian cancer.

Long Name

DNA [Cytosine-5-]-Methyltransferase 1

Alternate Names

CXXC9, MCMT

Gene Symbol

DNMT1

Additional DNMT1 Products

Product Documents for DNMT1 Antibody (1A9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DNMT1 Antibody (1A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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