Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # DNMT1/2061

Format

Azide and BSA Free

Concentration

1 mg/ml

View all DNMT1 Antibodies »

Product Specifications

Immunogen

Recombinant human DNMT1 protein fragment (around aa 767-912) (exact sequence is proprietary) (Uniprot: P26358)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

184 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07671).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free

Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482]

Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482]

Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Placenta stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).
Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482]

Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482]

Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Tonsil stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).

Applications for DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: DNMT1

DNA (cytosine-5)-methyltransferase 1 (DNMT1) plays a role in the regulation of tissue-specific cytosine residue methylation patterns that are established during development. Spec ifically, DNMT-1 interacts with Histone deacetylases and other methyltransferases to preferentially methylate hemimethylated DNA in coordination with the methylation of histones.

Improper methylation patterns have been linked to certain human tumors and developmental abnormalities. DNMT1 antibodies are useful tools for DNA methlyation research and studies on certian cancer.

Long Name

DNA [Cytosine-5-]-Methyltransferase 1

Alternate Names

CXXC9, MCMT

Gene Symbol

DNMT1

Additional DNMT1 Products

Product Documents for DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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