Dnmt2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB200-616

Novus Biologicals, part of Bio-Techne
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NB200-616
NB200-616SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Dnmt2 Antibodies »

Product Specifications

Immunogen

This antibody was raised against synthetic peptides corresponding amino acids 39-53 and 361-376 of mouse Dnmt2.

Reactivity Notes

Not yet tested in other species.

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dnmt2 Antibody

Western Blot: Dnmt2 Antibody [NB200-616]

Western Blot: Dnmt2 Antibody [NB200-616]

Western Blot: Dnmt2 Antibody [NB200-616] - Homogenized mouse brain cells. Image from verified customer review.
Western Blot: Dnmt2 Antibody [NB200-616]

Western Blot: Dnmt2 Antibody [NB200-616]

Western Blot: Dnmt2 Antibody [NB200-616] - analysis of Dnmt2 in mouse ES cell lysate using Dnmt2 antibodyat 2 ug/ml.
Western Blot: Dnmt2 Antibody [NB200-616]

Western Blot: Dnmt2 Antibody [NB200-616]

Western Blot: Dnmt2 Antibody [NB200-616] - Western blot analysis for Dnmt2a using NB200-616 at 2 ug/ml dilution against 10 ug of mouse testis lysates. A protein band of approximate mol. wt. of 42 kDa is detected.

Applications for Dnmt2 Antibody

Application
Recommended Usage

Western Blot

2 ug/ml
Application Notes
Western Blot: Detects a band of approximately 42 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NB200-616 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dnmt2

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Alternate Names

DMNT2, DNA (cytosine-5-)-methyltransferase 2, DNA (cytosine-5)-methyltransferase-like protein 2, DNA methyltransferase homolog HsaIIP, DNA methyltransferase-2, DNA MTase homolog HsaIIP, DNMT2, EC 2.1.1, EC 2.1.1.29, M.HsaIIP, MHSAIIP, PUMET, RNMT1, tRNA (cytosine-5-)-methyltransferase, tRNA aspartic acid methyltransferase 1

Entrez Gene IDs

1787 (Human); 13434 (Mouse)

Gene Symbol

TRDMT1

Additional Dnmt2 Products

Product Documents for Dnmt2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dnmt2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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