DNMT3B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18646
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for DNMT3B Antibody
Immunogen
Polyclonal antibody raised in rabbit against mouse DNMT3B (DNA methyltransferase 3B), using a KLH-conjugated synthetic peptide containing a sequence from the N-terminal part of the protein (1).
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for DNMT3B Antibody
Western Blot: DNMT3B Antibody [NBP3-18646]
Western Blot: DNMT3B Antibody [NBP3-18646] - Figure 2. Western blot analysis using the antibody directed against DNMT3B Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against DNMT3B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the MW marker (in kDa) is shown on the left.ELISA: DNMT3B Antibody [NBP3-18646]
ELISA: DNMT3B Antibody [NBP3-18646] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against mouse DNMT3B in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13,500.Applications for DNMT3B Antibody
Application
Recommended Usage
ELISA
1:100-1:500
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Whole Antiserum
Formulation
Whole antiserum
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: DNMT3B
Improper methylation patterns have been linked to certain human tumors and developmental abnormalities. DNMT3b antibodies are useful tools for DNA methlyation research and studies on certian cancer.
Long Name
DNA [Cytosine-5-]-Methyltransferase 3B
Alternate Names
ICF1, M.HsaIIIB
Gene Symbol
DNMT3B
UniProt
Additional DNMT3B Products
Product Specific Notices for DNMT3B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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