DOCK9 Antibody

Immunoprecipitation: DOCK9 Antibody [NB500-265]

Immunoprecipitation: DOCK9 Antibody [NB500-265] - Detection of human DOCK9 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DOCK9 antibody NB500-265 (lot NB500-265-2) used for IP at 6 ug per reaction. DOCK9 was also immunoprecipitated by a previous lot of this antibody (lot NB500-265-1) and rabbit anti-DOCK9 antibodies NB500-263 and NB500-264. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: DOCK9 Antibody [NB500-265] -

Absence of Zizimin proteins, but normal numbers of hematopoietic cells in KO mice. (A-B) Western blotting for Ziz2 (A) or Ziz3 (B) Protein lysates (30 μg/lane) were loaded & designated antibodies were reacted with the membranes. Replicated membranes were used for all antibodies. Zizimin proteins were absent in KO mice (8 weeks old, male). (C-F) The numbers of hematopoietic cells were counted after the hemolytic reaction (C-E) or flow cytometric analysis (F). No significant difference was observed among the groups (C-F). Three mice (8 weeks old, female) per group from three independent experiments (one mouse per group per experiment) were used. 2KO: Ziz2 KO. 3KO: Ziz3 KO. Image collected & cropped by CiteAb from the following publication (https://immunityageing.biomedcentral.com/articles/10.1186/s12979-015-0028-x), licensed under a CC-BY license. Not internally tested by Novus Biologicals.