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Dopa Decarboxylase/DDC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-56918

Novus Biologicals, part of Bio-Techne
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NBP1-56918

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to DDC (dopa decarboxylase, aromatic L-amino acid decarboxylase). The peptide sequence was selected from the N terminal of DDC (NP_000781). Peptide sequence EFRRRGKEMVDYVANYMEGIEGRQVYPDVEPGYLRPLIPAAAPQEPDTFE. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29789702).

Localization

Cytosol

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Dopa Decarboxylase/DDC Antibody

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918] - Reccomended Titration: 1 ug/ml Positive Control: Human 293T cell lysate
Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Immunohistochemistry-Paraffin: Dopa Decarboxylase/DDC Antibody [NBP1-56918] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.
Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918]

Western Blot: Dopa Decarboxylase/DDC Antibody [NBP1-56918] - HepG2 cell lysate, Antibody Titration: 5.0ug/ml

Applications for Dopa Decarboxylase/DDC Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

4-8 ug/ml

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dopa Decarboxylase/DDC

DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.

Alternate Names

AADC, DDC

Entrez Gene IDs

1644 (Human)

Gene Symbol

DDC

UniProt

Additional Dopa Decarboxylase/DDC Products

Product Documents for Dopa Decarboxylase/DDC Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dopa Decarboxylase/DDC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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