Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25444
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Canine, Feline
Predicted:
Chimpanzee (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1584
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Dopamine D2R/DRD2. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%), Porcine (88%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free
Immunohistochemistry-Paraffin: Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free [NBP3-25444] -
Immunohistochemistry-Paraffin: Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free [NBP3-25444] - Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cerebellum. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (NBP3-25444) diluted at 1:100. Red: MAP2, a neural marker, stained by MAP2 antibody [GT925] diluted at 1:250. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free [NBP3-25444] -
Western Blot: Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free [NBP3-25444] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine Receptor D2 antibody [HL1584] (NBP3-25444) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free [NBP3-25444] -
Immunocytochemistry/Immunofluorescence: Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free [NBP3-25444] - Dopamine Receptor D2 antibody [HL1584] detects Dopamine Receptor D2 protein at cell membrane by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 antibody [HL1584] (NBP3-25444) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .
Applications for Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Dopamine D2 R/DRD2
Long Name
Dopamine D2 Receptor
Alternate Names
D2DR, D2R, Dopamine D2R, DRD2
Gene Symbol
DRD2
Additional Dopamine D2 R/DRD2 Products
Product Documents for Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free
Product Specific Notices for Dopamine D2R/DRD2 Antibody (HL1584) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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