Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

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Product Summary for DORFIN Antibody

Immunogen

The immunogen for this product maps to a region between residue 600 and 650 of human Double Ring-Finger Protein using the numbering given in entry NP_056250.3 (GeneID 25897).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DORFIN Antibody

Immunoprecipitation: DORFIN Antibody [NBP1-71860]

Immunoprecipitation: DORFIN Antibody [NBP1-71860]

Immunoprecipitation: DORFIN Antibody [NBP1-71860] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Dorfin antibody used for IP at 6 mcg/mg lysate. Dorfin was also immunoprecipitated by another rabbit anti-Dorfin antibody which recognizes an upstream epitope.

Applications for DORFIN Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate
Application Notes
Western Blot Not recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DORFIN

RNF19A contains two RING-finger motifs and an IBR (in between RING fingers) motif. This protein is an E3 ubiquintin ligase that is localized in Lewy bodies (LBs), a characteristic neuronal inclusion in Parkinson's disease (PD) brains. This protein interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. This protein is found to bind and ubiquitylate synphilin 1 (SNCAIP), which is a interacting protein of alpha synuclein in neurons, and a major component of LB.The protein encoded this gene contains two RING-finger motifs and an IBR (in between RING fingers) motif. This protein is an E3 ubiquintin ligase that is localized in Lewy bodies (LBs), a characteristic neuronal inclusion in Parkinson's disease (PD) brains. This protein interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. This protein is found to bind and ubiquitylate synphilin 1 (SNCAIP), which is a interacting protein of alpha synuclein in neurons, and a major component of LB. Alternatively spliced transcript variants encoding the same protein have been reported.

Alternate Names

DKFZp566B1346, Dorfin, Double ring-finger protein, E3 ubiquitin-protein ligase RNF19A, EC 6.3.2, EC 6.3.2.-, p38, protein p38 interacting with transcription factor Sp1, ring finger protein 19, RING finger protein 19 isoform, ring finger protein 19Ap38 protein, ring-IBR-ring domain containing protein Dorfin, RNF19

Gene Symbol

RNF19A

UniProt

NP_056250.3 (Human)

Additional DORFIN Products

Product Documents for DORFIN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DORFIN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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