Skip to main content

DOT1L Antibody (3B6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15150

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
Loading...
NBP3-15150

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3B6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for DOT1L Antibody (3B6)

Immunogen

A synthesized peptide derived from human DOT1L. UniProt ID (Q8TEK3).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DOT1L Antibody (3B6)

Western Blot: DOT1L Antibody (3B6) [NBP3-15150]

Western Blot: DOT1L Antibody (3B6) [NBP3-15150]

Western Blot: DOT1L Antibody (3B6) [NBP3-15150] - Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, K562 whole cell lysate, SH-SY5Y whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate. All lanes: DOT1L antibody at 1:1500. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 165, 185 KDa. Observed band size: 185 KDa.

Applications for DOT1L Antibody (3B6)

Application
Recommended Usage

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: DOT1L

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Gene Symbol

DOT1L

Additional DOT1L Products

Product Documents for DOT1L Antibody (3B6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DOT1L Antibody (3B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...
Loading...
Loading...