DOT1L Antibody (6A6)
Novus Biologicals, part of Bio-Techne | Catalog # H00084444-M01
Key Product Details
Species Reactivity
Human, Feline
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 6A6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
DOT1L (NP_115871, 3 a.a. ~ 108 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLR
Reactivity Notes
Human. Other species not tested.
Specificity
DOT1L - DOT1-like, histone H3 methyltransferase (S. cerevisiae)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for DOT1L Antibody (6A6)
![Western Blot: DOT1L Antibody (6A6) [H00084444-M01]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-6A6-Western-Blot-H00084444-M01-img0005.jpg "Western Blot: DOT1L Antibody (6A6) [H00084444-M01]")
Western Blot: DOT1L Antibody (6A6) [H00084444-M01]
Western Blot: DOT1L Antibody (6A6) [H00084444-M01] - DOT1L monoclonal antibody (M01), clone 6A6. Analysis of DOT1L expression in LNCaP.![Immunocytochemistry/ Immunofluorescence: DOT1L Antibody (6A6) [H00084444-M01]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-6A6-Immunocytochemistry-Immunofluorescence-H00084444-M01-img0003.jpg "Immunocytochemistry/ Immunofluorescence: DOT1L Antibody (6A6) [H00084444-M01]")
Immunocytochemistry/ Immunofluorescence: DOT1L Antibody (6A6) [H00084444-M01]
Immunocytochemistry/Immunofluorescence: DOT1L Antibody (6A6) [H00084444-M01] - Analysis of monoclonal antibody to DOT1L on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: DOT1L Antibody (6A6) [H00084444-M01] - Detection limit for recombinant GST tagged DOT1L is approximately 0.03ng/ml as a capture antibody.
Applications for DOT1L Antibody (6A6)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: DOT1L
Long Name
Disruptor of Telomeric Silencing 1-like
Alternate Names
H3-K79-HMTase, KMT4
Entrez Gene IDs
84444 (Human)
Gene Symbol
DOT1L
OMIM
607375 (Human)
UniProt
Additional DOT1L Products
Product Documents for DOT1L Antibody (6A6)
Product Specific Notices for DOT1L Antibody (6A6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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