DOT1L Antibody (7A10H9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15446
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7A10H9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for DOT1L Antibody (7A10H9)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DOT1L (NP_115871.1). MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNT
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DOT1L Antibody (7A10H9)
![Western Blot: DOT1L Antibody (7A10H9) [NBP3-15446]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-7A10H9-Western-Blot-NBP3-15446-img0001.jpg "Western Blot: DOT1L Antibody (7A10H9) [NBP3-15446]")
Western Blot: DOT1L Antibody (7A10H9) [NBP3-15446]
Western Blot: DOT1L Antibody (7A10H9) [NBP3-15446] - Western blot analysis of extracts of various cell lines, using DOT1L Rabbit mAb (NBP3-15446) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunoprecipitation: DOT1L Antibody (7A10H9) [NBP3-15446]](https://resources.bio-techne.com/images/products/DOT1L-Antibody-7A10H9-Immunoprecipitation-NBP3-15446-img0002.jpg "Immunoprecipitation: DOT1L Antibody (7A10H9) [NBP3-15446]")
Immunoprecipitation: DOT1L Antibody (7A10H9) [NBP3-15446]
Immunoprecipitation: DOT1L Antibody (7A10H9) [NBP3-15446] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DOT1L antibody (NBP3-15446). Western blot was performed from the immunoprecipitate using DOT1L antibody (NBP3-15446) at a dilition of 1:1000.Applications for DOT1L Antibody (7A10H9)
Application
Recommended Usage
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DOT1L
Long Name
Disruptor of Telomeric Silencing 1-like
Alternate Names
H3-K79-HMTase, KMT4
Gene Symbol
DOT1L
Additional DOT1L Products
Product Documents for DOT1L Antibody (7A10H9)
Product Specific Notices for DOT1L Antibody (7A10H9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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