Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI6E7

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DOT1L Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1008-1424 of human DOT1L (NP_115871) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

164.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DOT1L Antibody (OTI6E7) - Azide and BSA Free

Western Blot: DOT1L Antibody (OTI6E7)Azide and BSA Free [NBP2-72298]

Western Blot: DOT1L Antibody (OTI6E7)Azide and BSA Free [NBP2-72298]

Western Blot: DOT1L Antibody (OTI6E7) - Azide and BSA Free [NBP2-72298] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DOT1L.
Immunohistochemistry: DOT1L Antibody (OTI6E7) - Azide and BSA Free [NBP2-72298]

Immunohistochemistry: DOT1L Antibody (OTI6E7) - Azide and BSA Free [NBP2-72298]

Immunohistochemistry: DOT1L Antibody (OTI6E7) - Azide and BSA Free [NBP2-72298] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DOT1L Antibody (OTI6E7) - Azide and BSA Free [NBP2-72298]

Immunohistochemistry: DOT1L Antibody (OTI6E7) - Azide and BSA Free [NBP2-72298]

Immunohistochemistry: DOT1L Antibody (OTI6E7) - Azide and BSA Free [NBP2-72298] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for DOT1L Antibody (OTI6E7) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DOT1L

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Gene Symbol

DOT1L

Additional DOT1L Products

Product Documents for DOT1L Antibody (OTI6E7) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DOT1L Antibody (OTI6E7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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