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DOT1L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-40845

Novus Biologicals, part of Bio-Techne
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NB100-40845

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Chemotaxis, Chip Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue1000 and 1050 of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23861670).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

185 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DOT1L Antibody

Western Blot: DOT1L Antibody [NB100-40845]

Western Blot: DOT1L Antibody [NB100-40845]

Western Blot: DOT1L Antibody [NB100-40845] - DOT1L is involved in UV-survival in mammalian cells. Fifty ug of whole cell extracts from MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for DOT1L. Asterisk indicates an unspecific cross-reacting band. Ten ug of fractions from histone acid-extraction performed on MEFWT or MEFDOT1L cells were resolved by SDS-PAGE and Western-blotted for H3, H3K79me1 or H3K79me3. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1003611), licensed under a CC-BY license.
Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845]

Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845]

Immunohistochemistry-Paraffin: DOT1L Antibody [NB100-40845] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-DOT1L used at a dilution of 1:200 (1ug/ml). Detection: DAB
Chromatin Immunoprecipitation: DOT1L Antibody [NB100-40845] - Chromatin from KOPN-8 cell line was immunoprecipitated with anti-DOT1L antibody and analyzed by DNA sequencing. The figure illustrates the peak distribution of DOT1L binding within a 250 Kb region of chromosome 10 as detected using anti-DOT1L antibody.

Applications for DOT1L Antibody

Application
Recommended Usage

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Chromatin Immunoprecipitation was reported in scientific literature (PMID:19734945). For ChIP-Seq: 4 ug/30 ug chromatin. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DOT1L

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Entrez Gene IDs

84444 (Human)

Gene Symbol

DOT1L

UniProt

Additional DOT1L Products

Product Documents for DOT1L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DOT1L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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