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DOT1L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-56641

Novus Biologicals, part of Bio-Techne
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NBP2-56641

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for DOT1L Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: LIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DOT1L Antibody

Western Blot: DOT1L Antibody [NBP2-56641]

Western Blot: DOT1L Antibody [NBP2-56641]

Western Blot: DOT1L Antibody [NBP2-56641] - Analysis in human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: DOT1L Antibody [NBP2-56641]

Immunocytochemistry/ Immunofluorescence: DOT1L Antibody [NBP2-56641]

Immunocytochemistry/Immunofluorescence: DOT1L Antibody [NBP2-56641] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Western Blot: DOT1L Antibody [NBP2-56641]

Western Blot: DOT1L Antibody [NBP2-56641]

Western Blot: DOT1L Antibody [NBP2-56641] - Analysis in human cell line BEWO.

Applications for DOT1L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

1:100 - 1:250
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DOT1L

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Gene Symbol

DOT1L

Additional DOT1L Products

Product Documents for DOT1L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DOT1L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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