Doublecortin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72042

Novus Biologicals, part of Bio-Techne
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NBP1-72042
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

IF/IHC, Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Doublecortin/DCX Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KTSANMKAPQS corresponding to internal region according to NP_835365.1, NP_835364.1, NP_001182482.1.

Specificity

This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.

Marker

Immature Neuronal Marker

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Doublecortin Antibody

Western Blot: Doublecortin Antibody [NBP1-72042]

Western Blot: Doublecortin Antibody [NBP1-72042]

Western Blot: Doublecortin Antibody [NBP1-72042] - staining of Mouse fetal Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody [NBP1-72042]

Immunocytochemistry/ Immunofluorescence: Doublecortin Antibody [NBP1-72042]

Immunocytochemistry/Immunofluorescence: Doublecortin Antibody [NBP1-72042] - Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP1-72042]

Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP1-72042]

Immunohistochemistry-Paraffin: Doublecortin Antibody [NBP1-72042] - Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
View 2 more images

Applications for Doublecortin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/mL

Immunohistochemistry-Paraffin

2 - 4 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.01 - 0.03 ug/mL
Application Notes
Western blot: Approx 45 kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3 kDa according to Mouse NP_001103692.1).
IHC: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and KNRK cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Doublecortin/DCX

In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The protein encoded by this gene is a cytoplasmic protein which appears to direct neuronal migration by regulating the organization and stability of microtubules. The encoded protein contains two doublecortin domains, which bind microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene are a cause of X-linked lissencephaly. Multiple transcript variants encoding at least three different isoforms have been found for this gene.

Long Name

Doublecortin

Alternate Names

DBCN, Doublecortex, Doublin, Lis-X, LISX

Entrez Gene IDs

1641 (Human); 13193 (Mouse); 84394 (Rat)

Gene Symbol

DCX

UniProt

NP_000546.2 (Human)

Additional Doublecortin/DCX Products

Product Documents for Doublecortin Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Doublecortin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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