DPEP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86087

Novus Biologicals, part of Bio-Techne
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NBP1-86087
NBP1-86087-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DPEP1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SLIGVEGGHSIDSSLGVLRALYQLGMRYLTLTHSCNTPWADNWLVDTGDSEPQSQGLSPFGQRVVKELNRLGVLIDLAHVSVATMKATLQLSRAPVIFSHSSAYSVCASRRN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DPEP1 Antibody

Western Blot: DPEP1 Antibody [NBP1-86087]

Western Blot: DPEP1 Antibody [NBP1-86087]

Western Blot: DPEP1 Antibody [NBP1-86087] - Analysis in control (vector only transfected HEK293T lysate) and DPEP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: DPEP1 Antibody [NBP1-86087]

Immunocytochemistry/ Immunofluorescence: DPEP1 Antibody [NBP1-86087]

Immunocytochemistry/Immunofluorescence: DPEP1 Antibody [NBP1-86087] - Staining human cell line Hep G2 shows positivity in nucleus and cell junctions. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DPEP1 Antibody [NBP1-86087]

Immunohistochemistry-Paraffin: DPEP1 Antibody [NBP1-86087]

Immunohistochemistry-Paraffin: DPEP1 Antibody [NBP1-86087] - Staining of human testis.
View 6 more images

Applications for DPEP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DPEP1

DPEP1 (EC 3.4.13.11) is a kidney membrane enzyme that hydrolyzes a variety of dipeptides and is implicated in renalmetabolism of glutathione and its conjugates, e.g., leukotriene D4 (Kozak and Tate, 1982 (PubMed 6122685)). DPEP1 isresponsible for hydrolysis of the beta-lactam ring of antibiotics, such as penem and carbapenem (Campbell et al., 1984(PubMed 6334084)). Earlier, beta-lactamase enzymes were thought to occur only in bacteria, where their probablefunction was in protecting the organisms against the action of beta-lactam antibiotics. These antibiotics exhibitselective toxicity against bacteria but virtual inertness against many eukaryotic cells (Adachi et al., 1990 (PubMed2303490)).(supplied by OMIM)

Long Name

Dipeptidase 1

Alternate Names

HRDP, Microsomal Dipeptidase, Renal Dipeptidase

Entrez Gene IDs

1800 (Human)

Gene Symbol

DPEP1

Additional DPEP1 Products

Product Documents for DPEP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DPEP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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