DPH2 Antibody (3F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00001802-M07

Novus Biologicals, part of Bio-Techne
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H00001802-M07
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DPH2 Antibodies »

Product Specifications

Immunogen

DPH2 (AAH16956, 1 a.a. ~ 489 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MESMFSSPAEAALQRETGVPGLLTPLPDLDGVYELERVAGFVRDLGCERVALQFPDQLLGDAVAVAARLEETTGSKMFILGDTAYGSCCVDVLGAEQAGAQALIHFGPACLSPPARPLPVAFVLRQRSVALELCVKAFEAQNPDPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPPPAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSG

Specificity

DPH2 - DPH2 homolog (S. cerevisiae) (3F7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DPH2 Antibody (3F7)

Western Blot: DPH2 Antibody (3F7) [H00001802-M07]

Western Blot: DPH2 Antibody (3F7) [H00001802-M07]

Western Blot: DPH2 Antibody (3F7) [H00001802-M07] - Analysis of DPH2 expression in transfected 293T cell line by DPH2 monoclonal antibody (M07), clone 3F7. Lane 1: DPH2 transfected lysate (Predicted MW: 52.1 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: DPH2 Antibody (3F7) [H00001802-M07]

Immunocytochemistry/ Immunofluorescence: DPH2 Antibody (3F7) [H00001802-M07]

Immunocytochemistry/Immunofluorescence: DPH2 Antibody (3F7) [H00001802-M07] - Analysis of monoclonal antibody to DPH2 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: DPH2 Antibody (3F7) [H00001802-M07] - Detection limit for recombinant GST tagged DPH2 is 3 ng/ml as a capture antibody.

Applications for DPH2 Antibody (3F7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DPH2

This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

diphthamide biosynthesis protein 2, Diphthamide biosynthesis protein 2 homolog-like 2, diptheria toxin resistance protein required for diphthamide biosynthesis-like 2, diptheria toxin resistance protein required for diphthamide biosynthesis-like 2(S. cerevisiae), DPH2 homolog, DPH2 homolog (S. cerevisiae), DPH2L2diphthamide biosynthesis-like protein 2, DPH2-like 2, DPH2-like 2 (S. cerevisiae), HsDph2

Entrez Gene IDs

1802 (Human)

Gene Symbol

DPH2

UniProt

AAH16956 (Human)

Additional DPH2 Products

Product Documents for DPH2 Antibody (3F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DPH2 Antibody (3F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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