DPH5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00051611-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DPH5 Antibodies »

Product Specifications

Immunogen

DPH5 (NP_001070862.1, 1 a.a. - 285 a.a.) full-length human protein. MLYLIGLGLGDAKDITVKGLEVVRRCSRVYLEAYTSVLTVGKEALEEFYGRKLVVADREEVEQEADNILKDADISDVAFLVVGDPFGATTHSDLVLRATKLGIPYRVIHNASIMNAVGCCGLQLYKFGETVSIVFWTDTWRPESFFDKVKKNRQNGMHTLCLLDIKVKEQSLENLIKGRKIYEPPRYMSVNQAAQQLLEIVQNQRIRGEEPAVTEETLCVGLARVGADDQKIAAGTLRQMCTVDLGEPLHSLIITGGSIHPMEMEMLSLFSIPENSSESQSINGL

Specificity

DPH5 - DPH5 homolog (S. cerevisiae),

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for DPH5 Antibody

Western Blot: DPH5 Antibody [H00051611-B01P]

Western Blot: DPH5 Antibody [H00051611-B01P]

Western Blot: DPH5 Antibody [H00051611-B01P] - Analysis of DPH5 expression in transfected 293T cell line by DPH5 polyclonal antibody. Lane 1: DPH5 transfected lysate(31.35 KDa). Lane 2: Non-transfected lysate.

Applications for DPH5 Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DPH5

This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CGI-30, Diphthamide biosynthesis methyltransferase, diphthine synthase, DPH5 homolog (S. cerevisiae), EC 2.1.1.98, HSPC143, MGC61450, NPD015, protein x 0011

Entrez Gene IDs

51611 (Human)

Gene Symbol

DPH5

UniProt

NP_001070862.1 (Human)

Additional DPH5 Products

Product Documents for DPH5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for DPH5 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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