DPPA4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48634

Novus Biologicals, part of Bio-Techne
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NBP2-48634
NBP2-48634-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DPPA4 Antibodies »

Product Summary for DPPA4 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: CQQLKLSSKGQKLDAYKRLCAFAYPNQKDFPSTAKEAKIRKSLQKKLKVEKGETSLQSSETHPPEVALPPVGEPPALENSTALLEGVNTVVVTTS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DPPA4 Antibody

Western Blot: DPPA4 Antibody [NBP2-48634]

Western Blot: DPPA4 Antibody [NBP2-48634]

Western Blot: DPPA4 Antibody [NBP2-48634] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line NTERA-2
Immunocytochemistry/ Immunofluorescence: DPPA4 Antibody [NBP2-48634]

Immunocytochemistry/ Immunofluorescence: DPPA4 Antibody [NBP2-48634]

Immunocytochemistry/Immunofluorescence: DPPA4 Antibody [NBP2-48634] - Staining of human cell line PC-3 shows localization to nucleus & nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DPPA4 Antibody [NBP2-48634]

Immunohistochemistry-Paraffin: DPPA4 Antibody [NBP2-48634]

Immunohistochemistry-Paraffin: DPPA4 Antibody [NBP2-48634] - Staining in human testis and endometrium tissues using anti-DPPA4 antibody. Corresponding DPPA4 RNA-seq data are presented for the same tissues.
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Applications for DPPA4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DPPA4

DPPA4, also known as Developmental pluripotency-associated protein 4, is a 33.5 kDa, 304 amino acid protein that is involved in inhibition of embryonic cells differentiation and maintenance of target genes' active epigenetic status. Current research is being conducted on diseases such as embryonal and other forms of carcinoma. The protein interacts with RPS6KB1, VIM, DPPA2, PTPRO, and SH3GL3. Pathways of the protein are unknown.

Long Name

Developmental Pluripotency Associated 4

Alternate Names

developmental pluripotency associated 4, developmental pluripotency-associated protein 4, FLJ10713,2410091M23Rik

Gene Symbol

DPPA4

Additional DPPA4 Products

Product Documents for DPPA4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DPPA4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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