DPPA4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98786
![Western Blot: DPPA4 Antibody [NBP2-98786]](https://resources.bio-techne.com/images/products/DPPA4-Antibody-Western-Blot-NBP2-98786-img0002.jpg "Western Blot: DPPA4 Antibody [NBP2-98786]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for DPPA4 Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human DPPA4.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for DPPA4 Antibody
Western Blot: DPPA4 Antibody [NBP2-98786]
Western Blot: DPPA4 Antibody [NBP2-98786] - Anti-DPPA4 rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).![Immunoprecipitation: DPPA4 Antibody [NBP2-98786]](https://resources.bio-techne.com/images/products/DPPA4-Antibody-Immunoprecipitation-NBP2-98786-img0001.jpg "Immunoprecipitation: DPPA4 Antibody [NBP2-98786]")
Immunoprecipitation: DPPA4 Antibody [NBP2-98786]
Immunoprecipitation: DPPA4 Antibody [NBP2-98786] - DPPA4 was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. Lane C: 0.5 mg Jurkat Whole Cell Lysate4 ul anti-DPPA4 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-DPPA4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 40 kDaApplications for DPPA4 Antibody
Application
Recommended Usage
Immunoprecipitation
4-8 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: DPPA4
Long Name
Developmental Pluripotency Associated 4
Alternate Names
developmental pluripotency associated 4, developmental pluripotency-associated protein 4, FLJ10713,2410091M23Rik
Gene Symbol
DPPA4
Additional DPPA4 Products
Product Documents for DPPA4 Antibody
Product Specific Notices for DPPA4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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