Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DR1 Antibodies »

Product Summary for DR1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human DR1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for DR1 Antibody

Western Blot: DR1 Antibody [NBP2-97557]

Western Blot: DR1 Antibody [NBP2-97557]

Western Blot: DR1 Antibody [NBP2-97557] - Anti-DR1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 19 kDa. Observed band size: 19 kDa
Immunocytochemistry/ Immunofluorescence: DR1 Antibody [NBP2-97557]

Immunocytochemistry/ Immunofluorescence: DR1 Antibody [NBP2-97557]

Immunocytochemistry/Immunofluorescence: DR1 Antibody [NBP2-97557] - Immunofluorescence staining of DR1 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human DR1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-97557]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-97557]

Immunohistochemistry-Paraffin: DR1 Antibody [NBP2-97557] - Immunochemical staining of human DR1 in human gallbladder with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
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Applications for DR1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:400

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DR1

DR1 encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. [provided by RefSeq]

Alternate Names

Down-regulator of transcription 1, down-regulator of transcription 1, TBP-binding (negative cofactor 2), NC2, NC2-beta, Negative cofactor 2-beta, protein Dr1, TATA-binding protein-associated phosphoprotein

Gene Symbol

DR1

Additional DR1 Products

Product Documents for DR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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