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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M3-P4B4

Format

BSA Free

Concentration

1 mg/ml

Product Summary for DRAM Antibody (M3-P4B4) - BSA Free

Immunogen

Peptide Sequence - EKDYVYHVVS (amino acids 192 - 201)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for DRAM Antibody (M3-P4B4) - BSA Free

Western Blot: DRAM Antibody (M3-P4B4) [NBP2-50379]
Immunocytochemistry/Immunofluorescence: DRAM Antibody (M3-P4B4) [NBP2-50379]
Immunohistochemistry: DRAM Antibody (M3-P4B4) - BSA Free [NBP2-50379]

Immunohistochemistry: DRAM Antibody (M3-P4B4) - BSA Free [NBP2-50379]

Immunohistochemistry: DRAM Antibody (M3-P4B4) [NBP2-50379] - Analysis of normal colon mucosa, primary tumour and lymph node metastasis sections.

Applications for DRAM Antibody (M3-P4B4) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): ELISA- Peptide immunogen; Western Blot- Hela cell extract (cytoplasmic fraction); ICC/IF - Hela Cells; IHC- Human hepatocellular carcinoma.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DRAM

DRAM is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4.; Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy.

Alternate Names

Damage-regulated autophagy modulator, DNA damage-regulated autophagy modulator protein 1, DNA-damage regulated autophagy modulator 1, DRAMFLJ11259

Gene Symbol

DRAM1

Additional DRAM Products

Product Documents for DRAM Antibody (M3-P4B4) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DRAM Antibody (M3-P4B4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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